RESUMO
The strain JPL-2, capable of degrading fenoxaprop-P-ethyl (FE), was isolated from the soil of a wheat field and identified as Rhodococcus ruber. This strain could utilize FE as its sole carbon source and degrade 94.6% of 100 mg L−1 FE in 54 h. Strain JPL-2 could also degrade other aryloxyphenoxy propanoate (AOPP) herbicides. The initial step of the degradation pathway is to hydrolyze the carboxylic acid ester bond. A novel esterase gene feh, encoding the FE-hydrolyzing carboxylesterase (FeH) responsible for this initial step, was cloned from strain JPL-2. Its molecular mass was approximately 39 kDa, and the catalytic efficiency of FeH followed the order of FE > quizalofop-P-ethyl > clodinafop-propargyl > cyhalofop-butyl > fluazifop-P-butyl > haloxyfop-P-methyl > diclofop-methy, which indicated that the chain length of the alcohol moiety strongly affected the hydrolysis activity of the FeH toward AOPP herbicides.
Assuntos
Carboxilesterase/genética , Carboxilesterase/metabolismo , Herbicidas/metabolismo , Oxazóis/metabolismo , Propionatos/metabolismo , Rhodococcus/isolamento & purificação , Rhodococcus/metabolismo , Biotransformação , Clonagem Molecular , Análise por Conglomerados , Carboxilesterase/química , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Dados de Sequência Molecular , Peso Molecular , Filogenia , /genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Rhodococcus/enzimologia , Rhodococcus/genética , Análise de Sequência de DNA , Microbiologia do Solo , Especificidade por Substrato , Triticum/crescimento & desenvolvimentoRESUMO
116 cases of facial pigmented spots were treated by the needle-embedding method in Uterus (MA-TF). EarShenmen (MA-TF1), Endocrine (MA-IC3) and Lung (MA-IC1) as the main points and the additional points selected based upon pattern identification. All cases were cured.